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Automatically clear input sequence
In order to analyse a protein sequence you would normally copy it from a document or a website such as UniProt, using the clipboard. In this case the input sequence could contain such symbols as numbers, brackets and so on. Do not waste your time cleaning up your input! This web tool will make it for you automatically by removing those symbols that do not comply with the protein sequence one-letter code. Just click on "SEQ>" below and then paste your data from the clipboard. Your sequence will be cleaned and grouped by 10 amino acids. Now you can submit the sequence for an analysis on this website, or save it for your future analyses.
Dual output format
This web tool can present a protein sequence in two indexed formats both of which group amino acids in blocks of ten. The default format will use a space character to separate between the blocks. However, you can choose to switch to a more compact representation (especially useful for long sequences) using a vertical line separator. You can easily whitch between formats using and buttons in the menu.
Easy access to all functions
By hovering mouse over the menu button and selecting a desired option, allows you to (i) switch between the two sequence formats, (ii) go back to the sequence input page, (iii) save the current view of the sequence as a PNG file, (iv) print the page, (v) change the color schema or (vi) search for a pattern.
Sometimes it is useful to highlight amino acids of a certain type to analyze their quantity and visualize their locations within the protein sequence. To facilitate such visual analysis of the protein primary structure we made amino acids symbols clickable. In just one click you can select/unselect amino acids of a certain type to visualize that type's distribution across the sequence.
Analyze multiple protein regions in one go
You may want to analyze properties of a specific region of the sequence. For that you just need to click on the starting symbol and move the cursor along the sequence while keeping the mouse button pressed, then release the mouse button at the end of your selection. Repeat the same operation as many times as required to select multiple regions. Our web tool will automatically calculate and display for you specific properties (please see below) of the selected regions.
Calculate properties of selected regions
Upon selection of those regions of the sequence that you would like to analyze, our web tool automatically reports on their characteristics. These include the length of the selected regions, molecular weight, monoisotopic weight, number of positively and negatively charged amino acids, extinction coefficient, etc.
Access to structural data from UniProt
If the protein sequence that you have loaded exists in the UniProt database, its structural and functional information will be loaded and displayed for you. Just by pointing the cursor and clicking the button you will be able to see the location of different parts of the protein in the cytoplasm or lumen. Our tool also displays information on the secondary structure, domains, repeats, mutations or modifications. You need just press button.
Annotate, print out, save and share your analyses
You can add custom annotations underneath the sequence line. Our tool allows you to print out and/or save as PNG images different views of the analyzed sequence, representing different stages of you analysis. These print outs and images can then be shared with your collaborators. Alternatively, you can just send them the URL from the browser location input and they will see exactly the same representation of the sequence as you do, with your comments, highlights and selections.